又一篇SCI論文！證明了日糧中添加膽汁酸對(duì)舌鰨肝臟健康的有益影響！

Dietary Supplementation of Bile Acids in Tongue Sole (Cynoglossus semilaevis): A Promising Strategy to Improve Hepatic Health Status

半滑舌鰨日糧中補(bǔ)充膽汁酸：一個(gè)有希望改善肝臟健康狀況的策略

作者：王升鵬、張偉、曹愛(ài)智、潘中超、劉太亮

A 10-week feeding trial was conducted to investigate the beneficial effects of bile acids (BAs) on hepatic health status in tongue sole (Cynoglossus semilaevis). Three experimental diets were prepared with different levels of BA inclusion in the commercial basal diet: control group (CT: 0 mg/kg basal diet), low-level group (BA1: 300 mg/kg basal diet), and high-level group (900 mg/kg basal diet). At the end of the feeding trial, growth performance and survival rate were measured, as well as numerous physiological and biochemical parameters of the liver, including four hepatic function indices, five antioxidant indices, five lipid metabolism parameters, and three digestive enzyme activities. Moreover,the mRNA expression levels of three growth-related genes and three immune-related genes in the liver were assayed. Results showed that growth performance and survival were substantially improved in both low- and high-level BA inclusive groups. Further, the tested liver physiological and biochemical parameters combined with the quantitative real-time PCR results revealed enhanced antioxidant capacity, energy metabolism, digestive ability, and immune response. Finally, these findings provide a wider spectrum of the beneficial effects of dietary BAs on liver health in tongue sole.

譯文：

為研究膽汁酸對(duì)半滑舌鰨（Cynoglossus semilaevis）肝臟健康狀況的有益影響，進(jìn)行了為期10周的喂養(yǎng)試驗(yàn)。在商業(yè)基礎(chǔ)日糧中制備了三種含有不同水平膽汁酸的試驗(yàn)日糧：對(duì)照組（0 mg/kg）、低水平組（300 mg/kg）和高水平組（900 mg/kg）。喂食試驗(yàn)結(jié)束后，測(cè)量生長(zhǎng)性能和存活率,以及肝臟的各項(xiàng)生理生化參數(shù)，包括四項(xiàng)肝功能指標(biāo)、五項(xiàng)抗氧化指標(biāo)、五項(xiàng)脂代謝參數(shù)和三項(xiàng)消化酶活性。此外，還檢測(cè)了肝臟中三個(gè)生長(zhǎng)相關(guān)基因和三個(gè)免疫相關(guān)基因的mRNA表達(dá)水平。結(jié)果表明，低水平膽汁酸組和高水平膽汁酸組的生長(zhǎng)性能和存活率均顯著提高。此外，檢測(cè)的肝臟生理生化參數(shù)結(jié)合實(shí)時(shí)定量PCR結(jié)果顯示：抗氧化能力、能量代謝、消化能力和免疫反應(yīng)增強(qiáng)。最后，這些發(fā)現(xiàn)提供了更廣泛的日糧添加膽汁酸對(duì)舌鰨肝臟健康的有益影響。